The Mouse BDNF (Brain-Derived Neurotrophic Factor) ELISA Kit is specifically designed for the accurate quantification of BDNF levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.BDNF is a key protein in the brain that is involved in promoting the growth, survival, and differentiation of neurons. It plays a critical role in various neurological processes, including learning, memory, and mood regulation. Dysregulation of BDNF has been implicated in several neurological disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse BDNF ELISA Kit is an essential tool for researchers working in the field of neuroscience and studying the role of BDNF in brain function and neurodegenerative diseases.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 2 vials, 20 mL | 96T*5: 10 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
87-98
86-96
94-109
Average (%)
92
91
101
1:4
Range (%)
98-110
80-92
92-107
Average (%)
103
86
100
1:8
Range (%)
97-110
84-97
97-111
Average (%)
105
91
103
1:16
Range (%)
99-114
82-97
95-109
Average (%)
107
88
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
93-109
100
Cell Culture Media (n=8)
95-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.29
313.76
735.3
91.27
342.33
679.92
Standard deviation
6.08
13.18
43.46
5.84
23.79
59.09
C V (%)
6.12
4.2
5.91
6.4
6.95
8.69
Uniport ID:
P21237
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse BDNF in samples. No significant cross-reactivity or interference between Mouse BDNF and analogues was observed
Storage:
2-8℃
Research Area:
Cancer,Cardiovascular,Metabolism,Neuroscience
Konstantinos Mesiakaris et al.
Cannabidiol Modulation of Nicotine-Induced Toxicity: Assessing Effects on Behavior, Brain-Derived Neurotrophic Factor, and Oxidative Stress in C57BL/6 Male Mice
