The Mouse Beta TG (Beta Thromboglobulin) ELISA Kit is specifically designed for the precise measurement of beta thromboglobulin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent choice for a variety of research applications.Beta thromboglobulin is a key protein involved in platelet activation and aggregation, playing a crucial role in the process of blood clot formation. Abnormal levels of beta thromboglobulin have been associated with various thrombotic disorders, making it a valuable biomarker for studying these conditions and developing potential treatment strategies.Overall, the Mouse Beta TG (Beta Thromboglobulin) ELISA Kit is a powerful tool for researchers looking to investigate the role of beta thromboglobulin in various physiological and pathological processes in mice.
Product Name:
Mouse beta-TG (beta-Thromboglobulin) ELISA Kit
SKU:
MOES01627
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 3 vials, 20 mL | 96T*5: 15 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
98-114
97-107
91-109
Average (%)
106
102
99
1:4
Range (%)
96-111
80-94
95-109
Average (%)
104
87
101
1:8
Range (%)
96-109
83-93
98-112
Average (%)
101
88
104
1:16
Range (%)
97-114
87-98
94-108
Average (%)
104
92
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
84-99
90
Cell Culture Media (n=8)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.99
50.4
229.89
24.85
54.93
208.15
Standard deviation
1.34
3.07
11.03
2.1
4.05
14.26
C V (%)
5.35
6.1
4.8
8.44
7.38
6.85
Uniport ID:
Q9EQI5
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Mouse βTG/PBP/CXCL7/NAP2 in samples. No significant cross-reactivity or interference between Mouse βTG/PBP/CXCL7/NAP2 and analogues was observed
