The Mouse BNP (Brain Natriuretic Peptide) ELISA Kit is specifically designed for the accurate measurement of BNP levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing dependable and consistent results for a variety of research applications.BNP is a vital peptide hormone that plays a key role in regulating blood pressure and fluid balance. It is predominantly produced and released by the heart in response to increased pressure and volume overload, making it an important biomarker for cardiovascular diseases such as heart failure and hypertension. With its reliable performance and broad utility, the Mouse BNP ELISA Kit is an essential tool for researchers studying cardiovascular health and developing innovative therapies for related conditions.
Product Name:
Mouse BNP (Brain Natriuretic Peptide) ELISA Kit
SKU:
MOES00783
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
