Mouse Bone Morphogenetic Protein 8B (BMP8B) ELISA Kit
The Mouse Bone Morphogenetic Protein 8B (BMP8B) ELISA Kit is a valuable tool for researchers looking to accurately measure levels of BMP8B in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for precise and reproducible results for a variety of experimental needs.BMP8B is a key player in bone and cartilage development, as well as in metabolic regulation and energy balance. It is involved in various physiological processes such as bone growth, thermogenesis, and metabolism, making it a critical factor in understanding conditions like osteoporosis, obesity, and metabolic disorders.
By providing a reliable method for quantifying BMP8B levels, this ELISA kit offers researchers the opportunity to further explore the role of this important protein in health and disease, ultimately paving the way for the development of novel therapeutic approaches.
Product Name:
Mouse Bone Morphogenetic Protein 8B (BMP8B) ELISA Kit
Product Code:
MODL00163
Size:
96 Assays
Target:
BMP8B
Synonyms:
BMP8-B, OP2, Osteogenic Protein 2
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
