The Mouse C4 (Complement Component 4) ELISA Kit is a highly reliable assay designed for the precise and quantitative measurement of C4 levels in a variety of mouse biological samples. Complement Component 4 is an essential protein involved in the complement system, playing a pivotal role in immune responses, inflammation, and clearance of pathogens from the body. Ensuring exceptional sensitivity and specificity, our Mouse C4 ELISA Kit delivers accurate and reproducible results, providing researchers with valuable insights into the role of C4 in immune modulation and disease mechanisms. Manufactured under stringent quality control measures, this kit offers robust performance and user-friendly protocols, making it an excellent choice for advancing research in the fields of immunology and inflammation.
Product Name:
Mouse C4 (Complement Component 4) ELISA Kit
SKU:
AEES00215
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
