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APC Anti-Mouse CD162 Antibody [4RA10] (AGEL0841)

SKU:
AGEL0841
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Applications:
FC
€319 - €709
Frequently bought together:

Description

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Product Title:APC Anti-Mouse CD162 Antibody [4RA10]
SKU:AGEL0841
Size:50 Tests, 100 Tests, 200 Tests
Clone No:4RA10
Synonyms:P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG1, κ
Isotype Control:APC Rat IgG1, κ Isotype Control[HRPN]
Form:Liquid
Conjugation:APC
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:Spectrum Image
Swissprot:Q62170
Gene ID:20345
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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