Mouse CD223/LAG3 Recombinant Protein (C-His) (GXRP10609)

Mouse CD223/LAG3 Recombinant Protein (C-His)

Mouse CD223/LAG3 Recombinant Protein (C-His) is a recombinant protein expressed in mammalian cells, spanning Gly24-Leu442, with a C-terminal His tag. Predicted molecular weight: 48.14 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Lymphocyte activation gene 3 protein is a ~56 kDa protein. Inhibitory receptor on antigen activated T-cells. For research use only.

Delivers inhibitory signals upon binding to ligands, such as MHC class II, its main ligand present at the surface of antigen-presenting cells (APCs), and FGL1, which is secreted by hepatocytes and certain types of tumor cells. Ligand-binding initiates a signaling that inhibits the T-cell receptor (TCR) in the immunological synapse, preventing T-cell activation. Mechanistically, ligand-binding promotes (1) ubiquitination of the KIEELE motif, unleashing the RRFSALE motif from the membrane and (2) leading to the formation of condensates with the TCR component CD3E, thereby disrupting the association between CD3E and LCK and preventing TCR activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1.