Description
Mouse CDKN1A Recombinant Protein (N-GST & C-His)
Mouse CDKN1A Recombinant Protein (N-GST & C-His) is a recombinant protein expressed in E. coli, spanning Ser2-Pro159, with an N-terminal GST tag and a C-terminal His tag. Predicted molecular weight: 45.83 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Cyclin-dependent kinase inhibitor 1 (p21/CDKN1A) is a ~17 kDa protein. May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. For research use only.
Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.
| Product Name: | Mouse CDKN1A Recombinant Protein (N-GST & C-His) |
| SKU: | GXRP07798 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein, p21, Cdkn1a, Cip1, Waf1 |
| Tag: | N-GST & C-His |
| Expression Host: | E. coli |
| Protein Length: | Ser2-Pro159 |
| Calculated MW: | 45.83 kDa |
| UniProt: | P39689 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
