Mouse Cholecystokinin A Receptor (CCKAR) ELISA Kit
The Mouse Cholecystokinin-A Receptor (CCKAR) ELISA Kit is specially designed for the precise measurement of CCKAR levels in mouse samples, including serum, plasma, and tissue culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable tool for various research applications.CCKAR is a key receptor involved in mediating the effects of cholecystokinin, a peptide hormone that regulates various physiological processes such as digestion and appetite. Dysregulation of CCKAR has been implicated in conditions like gastrointestinal disorders, obesity, and mood disorders, highlighting its importance as a potential therapeutic target.
This ELISA kit enables researchers to study the role of CCKAR in health and disease, providing valuable insights for developing novel treatments and interventions. With its reliable performance and ease of use, the Mouse CCKAR ELISA Kit is an essential tool for advancing scientific understanding in the field of molecular biology and pharmacology.
Product Name:
Mouse Cholecystokinin A Receptor (CCKAR) ELISA Kit
Product Code:
MODL00223
Size:
96 Assays
Target:
CCKAR
Synonyms:
CCK-A, CCK1, CCKRA, CCK1R, Cholecystokinin Type 1 Receptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
