The Mouse CKM (Creatine Kinase-Muscle) ELISA Kit is specifically designed for the accurate and reliable detection of CKM levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CKM is an important enzyme found predominantly in muscle tissue, playing a key role in energy metabolism and muscle contraction. Elevated CKM levels can indicate muscle damage or disease, making it a valuable biomarker for studying muscular disorders, exercise-induced muscle damage, and monitoring the effects of potential treatments.With this Mouse CKM ELISA Kit, researchers can confidently measure CKM levels in mouse samples with ease and precision, enhancing their understanding of muscle physiology and pathology.
Product Name:
Mouse CKM (Creatine Kinase, Muscle) ELISA Kit
SKU:
MOES00917
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
