The Mouse Coagulation Factor II (F2) ELISA Kit is specifically designed for the precise detection of coagulation factor II levels in mouse serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring consistent and dependable results for a variety of research purposes.Coagulation factor II, also known as prothrombin, is a key protein in the blood clotting process. It plays a crucial role in maintaining hemostasis and preventing excessive bleeding.
Dysregulation of coagulation factor II can lead to various bleeding disorders and thrombotic events, making it a valuable biomarker for studying these conditions and potential treatment options. With its superior performance and accuracy, the Mouse Coagulation Factor II (F2) ELISA Kit is an essential tool for researchers looking to explore the role of coagulation factor II in a range of physiological and pathological processes.
Product Name:
Mouse Coagulation Factor II (F2) ELISA Kit
Product Code:
MODL00439
Size:
96 Assays
Target:
F2
Synonyms:
FII, TM, PT, Thrombin, Prothrombin, Pro-Thrombin, Activation peptide fragment 1, Activation peptide fragment 2
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
