The Mouse CRP (C-reactive protein) CLIA Kit is specifically designed for the quantitative measurement of CRP levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.CRP is a well-known marker of inflammation and is commonly used in the diagnosis and monitoring of a wide range of inflammatory conditions. Elevated levels of CRP have been associated with various diseases, including cardiovascular disease, autoimmune disorders, and infections, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.
Overall, the Mouse CRP CLIA Kit provides researchers with a reliable tool for investigating the role of CRP in various disease processes and developing new strategies for diagnosis and treatment.
Product Name:
Mouse CRP (C-Reactive Protein) CLIA Kit
SKU:
MOES00048
Target:
Mouse CRP (C-Reactive Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CRP. You can calculate the concentration of Mouse CRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
97-111
96-109
Average (%)
96
103
102
1:4
Range (%)
100-115
101-113
103-117
Average (%)
107
107
109
1:8
Range (%)
86-97
85-98
95-108
Average (%)
91
90
102
1:16
Range (%)
95-110
100-115
92-105
Average (%)
101
106
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
269.29
777.88
1679.49
249.13
709.95
1712.51
Standard deviation
31.88
81.21
143.26
25.83
50.55
153.61
C V (%)
11.84
10.44
8.53
10.37
7.12
8.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CRP in samples. No significant cross-reactivity or interference between Mouse CRP and analogues was observed.
