The Mouse CTSB (Cathepsin B) ELISA Kit is specifically designed for quantitative detection of cathepsin B levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Cathepsin B is a key protease involved in various physiological processes, including protein degradation, antigen processing, and cell migration. Dysregulation of cathepsin B has been linked to a range of diseases, such as cancer, inflammation, and neurodegenerative disorders, highlighting its potential as a valuable biomarker for disease diagnosis and therapeutic development. The Mouse CTSB ELISA Kit is a valuable tool for researchers looking to study cathepsin B dynamics in mouse models, providing a comprehensive understanding of its role in disease progression and potential therapeutic interventions.
Product Name:
Mouse CTSB (Cathepsin B) ELISA Kit
SKU:
MOES01703
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
