The Mouse CTSK (Cathepsin K) ELISA Kit is specifically designed for the accurate quantification of Cathepsin K levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Cathepsin K is a critical enzyme involved in bone resorption and remodeling, making it a key target for studying conditions such as osteoporosis, arthritis, and other bone-related disorders. By measuring Cathepsin K levels, researchers can gain valuable insights into disease progression and potential therapeutic interventions.With its advanced technology and user-friendly protocol, the Mouse CTSK ELISA Kit from Assay Genie is an essential tool for investigating the role of Cathepsin K in various biological processes and disease conditions. Trust in the accuracy and precision of this kit to advance your research and discovery efforts.
Product Name:
Mouse CTSK (Cathepsin K) ELISA Kit
SKU:
MOES00820
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
