The Mouse CTSL (Cathepsin L) ELISA Kit is a powerful tool for the accurate measurement of Cathepsin L levels in mouse serum, plasma, and tissue homogenates. With excellent sensitivity and specificity, this kit delivers dependable and consistent results, making it a valuable asset for various research applications. Cathepsin L is a critical enzyme involved in numerous biological processes, including protein degradation, antigen processing, and tissue remodeling. Its dysregulation has been linked to various pathologies, such as cancer, inflammation, and metabolic disorders, underscoring its importance as a potential therapeutic target and diagnostic marker. With the Mouse CTSL ELISA Kit, researchers can explore the intricate role of Cathepsin L in health and disease, paving the way for innovative treatments and diagnostic strategies. Trust in this kit for accurate and precise measurements that drive impactful discoveries in the field of biomedical research.
Product Name:
Mouse CTSL (Cathepsin L) ELISA Kit
SKU:
MOES00821
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
