The Mouse CXCR3 (C-X-C chemokine receptor 3) CLIA Kit is a highly reliable and sensitive tool for the precise measurement of CXCR3 levels in mouse serum, plasma, and cell culture supernatants. This kit is designed to provide accurate and reproducible results, making it ideal for a variety of research applications.CXCR3 is a critical chemokine receptor involved in immune responses and inflammatory processes. It plays a key role in regulating the trafficking of immune cells and has been implicated in various diseases, including autoimmune disorders and cancer.
By detecting and quantifying CXCR3 levels, researchers can gain valuable insights into the role of this receptor in different biological processes and disease states, potentially leading to the development of novel therapeutic strategies.Overall, the Mouse CXCR3 CLIA Kit offers a powerful tool for studying the function and regulation of CXCR3 in mouse models, providing researchers with a valuable resource for advancing their research in immunology, inflammation, and related fields.
Product Name:
Mouse CXCR3 (CXC-chemokine receptor 3) CLIA Kit
SKU:
MOES00209
Target:
Mouse CXCR3 (CXC-chemokine receptor 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCR3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CXCR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CXCR3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CXCR3. You can calculate the concentration of Mouse CXCR3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
88-102
99-114
Average (%)
103
96
106
1:4
Range (%)
85-98
86-100
97-109
Average (%)
91
91
103
1:8
Range (%)
85-99
86-99
98-112
Average (%)
91
91
104
1:16
Range (%)
90-101
85-100
98-112
Average (%)
96
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
90-106
96
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.25
279.68
778.84
98.17
273.46
729.46
Standard deviation
9.1
21.09
65.5
12.07
24.28
60.69
C V (%)
8.65
7.54
8.41
12.29
8.88
8.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CXCR3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CXCR3 in samples. No significant cross-reactivity or interference between Mouse CXCR3 and analogues was observed.
