The Mouse CYP1A2 (P450 Cytochrome P450 1A2) ELISA Kit is specifically designed for the accurate measurement of mouse CYP1A2 levels in various biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a wide range of research applications. Cytochrome P450 1A2 is an important enzyme involved in the metabolism of various drugs and environmental toxins in the liver. It plays a crucial role in the detoxification process and can also contribute to the activation of procarcinogens.
Monitoring CYP1A2 levels can provide valuable insights into drug metabolism, toxicology studies, and pharmacogenomics research. By accurately quantifying mouse CYP1A2 levels, researchers can better understand the role of this enzyme in drug metabolism and toxicity, ultimately leading to the development of safer and more effective therapies. This ELISA kit is an essential tool for studying the function and regulation of CYP1A2, making it a valuable asset for any laboratory conducting research in the fields of pharmacology, toxicology, and drug development.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-101%
88-98%
80-99%
EDTA Plasma (n = 5)
87-96%
92-101%
86-99%
Heparin Plasma (n = 5)
86-103%
83-96%
84-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
91-101
96
EDTA Plasma (n = 5)
86-95
91
Heparin Plasma (n = 5)
87-103
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
