Description
Mouse Cyp21 Recombinant Protein (N-His)
Mouse Cyp21 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu30-Arg476, with an N-terminal His tag. Predicted molecular weight: 52.98 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Steroid 21-hydroxylase (Cyp21) is a ~55 kDa protein. A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. For research use only.
Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
| Product Name: | Mouse Cyp21 Recombinant Protein (N-His) |
| SKU: | GXRP07778 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | Steroid 21-hydroxylase, 1.14.14.16, 21-OHase, Cytochrome P-450c21, Cytochrome P450 21, Cytochrome P450 XXI, Cytochrome P450-C21, Cyp21, Cyp21a-1, Cyp21a1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu30-Arg476 |
| Calculated MW: | 52.98 kDa |
| UniProt: | P03940 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
