The Mouse DAO (Diamine Oxidase) ELISA Kit is specifically designed for the precise measurement of diamine oxidase levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Diamine oxidase is an important enzyme involved in the breakdown of histamine and other biogenic amines, playing a vital role in maintaining gut health and immune function. Dysregulation of DAO has been linked to conditions such as allergy, inflammation, and gastrointestinal disorders, making it a valuable biomarker for studying these diseases and developing potential treatments.Overall, the Mouse DAO ELISA Kit from Assay Genie provides researchers with a reliable tool for investigating the role of diamine oxidase in health and disease in mouse models.
Product Name:
Mouse DAO (Diamine Oxidase) ELISA Kit
SKU:
MOES00959
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
2.35 ng/mL
Detection range:
3.91-250 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
