The Mouse Defensin Beta-1 (DEFB1) ELISA Kit is a high-quality assay designed for the precise measurement of defensin beta-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Defensin beta-1 is a critical antimicrobial peptide that plays a vital role in the immune response, protecting against bacterial and fungal infections. Studies have shown that defensin beta-1 also has immunomodulatory and anti-inflammatory properties, making it a valuable marker for investigating immune responses and inflammatory conditions in mouse models.
Whether studying host defense mechanisms, infection pathways, or immune system regulation, the Mouse Defensin Beta-1 (DEFB1) ELISA Kit provides researchers with a reliable tool to advance their understanding of defensin beta-1 biology and its implications for disease pathology and therapeutic interventions.
Product Name:
Mouse Defensin Beta 1 (DEFb1) ELISA Kit
Product Code:
MODL00366
Size:
96 Assays
Target:
DEFb1
Synonyms:
B-DF1, DEFB1, BD1, DEF-B1, DEFB101, HBD1
Detection Method:
Sandwich
Range:
3.125-200pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
