The Mouse DKK3 (Dickkopf-related protein 3) ELISA Kit is specifically designed for the accurate detection of DKK3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.DKK3 is a key player in regulating the Wnt signaling pathway and has been implicated in various diseases including cancer, cardiovascular diseases, and neurodegenerative disorders. By studying DKK3 levels, researchers can gain insights into the underlying mechanisms of these conditions and potentially develop targeted therapies.With its user-friendly format and reliable performance, the Mouse DKK3 ELISA Kit from Assay Genie is a valuable tool for researchers looking to study the role of DKK3 in disease pathology and therapeutic interventions.
Product Name:
Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit
SKU:
MOES00977
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
