The Mouse EGF (Epidermal Growth Factor) ELISA Kit is specifically designed for the precise quantification of EGF levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent tool for various research applications.EGF is a key growth factor that plays a critical role in cell proliferation, differentiation, and survival. It is involved in various physiological processes, including wound healing, tissue regeneration, and development. Dysregulation of EGF signaling has been implicated in diseases such as cancer and inflammatory disorders, underscoring the importance of studying EGF levels for medical research and therapeutic development.Order the Mouse EGF ELISA Kit today to advance your research and gain valuable insights into the role of EGF in mouse biology.
Product Name:
Mouse EGF (Epidermal growth factor) ELISA Kit
SKU:
MOES00645
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
