Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit
The Mouse EGFL7 (EGF-Like Domain Protein Multiple 7) ELISA Kit is a powerful tool for the precise measurement of EGFL7 levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides dependable and consistent results, making it an excellent choice for a variety of research studies.EGFL7 is a key protein involved in angiogenesis and vascular development, playing a critical role in regulating blood vessel formation and growth. Its expression has been linked to various diseases including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for disease research and drug development efforts.
By using the Mouse EGFL7 ELISA Kit, researchers can accurately quantify EGFL7 levels in biological samples, enabling a better understanding of its role in disease pathogenesis and potential therapeutic interventions. This kit is a valuable asset for scientists conducting studies in the fields of vascular biology, cancer research, and cardiovascular medicine.
Product Name:
Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit
Product Code:
MODL00407
Size:
96 Assays
Target:
EGFL7
Synonyms:
ZNEU1, MEGF7, Zneu1, Vascular endothelial statin, EGF Like Domain Containing 7, Epidermal growth factor-like protein 7, Multiple epidermal growth factor-like domains protein 7
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
