The Mouse Eotaxin 1 ELISA Kit is designed for the quantitative detection of Mouse Eotaxin 1 levels in various biological samples. Eotaxin 1 is a biologically active molecule with established roles in cell biology, immunology, neuroscience pathways. It participates in key cellular and molecular processes that regulate physiological homeostasis, and dysregulation of its expression or activity has been associated with pathological states across multiple disease contexts. The precise quantification of Mouse Eotaxin 1 in biological samples is critical for elucidating its mechanistic contributions to disease pathogenesis, identifying clinically relevant biomarker associations, and supporting the development of novel diagnostic tools and targeted therapeutic interventions. Assay Genie's Mouse Eotaxin 1 ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 9.38 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma, and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Mouse Eotaxin 1 ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Mouse Eotaxin 1 ELISA Kit
SKU:
AEES03506
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
84-97
85-99
95-112
Average (%)
91
92
103
1:4
Range (%)
86-98
86-100
92-104
Average (%)
92
92
99
1:8
Range (%)
81-92
82-97
94-106
Average (%)
86
89
99
1:16
Range (%)
82-96
83-97
93-105
Average (%)
87
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
85-96
90
Cell Culture Media (n=8)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.1
114.27
351.62
45.42
109.93
375.72
Standard deviation
2.96
5.61
15.01
2.72
5.49
12.06
C V (%)
6.28
4.91
4.27
5.98
4.99
3.21
Uniport ID:
P48298
Sample type & Sample volume:
100 μL
Specificity:
This kit recognizes Mouse Eotaxin 1/CCL11 in samples.No significant cross-reactivity or interference between Mouse Eotaxin 1/CCL11 and analogues was observed
