Mouse F-Box And Leucine Rich Repeat Protein 3 (FBXL3) ELISA Kit
The Mouse F-Box and Leucine-Rich Repeat Protein 3 (FBXL3) ELISA Kit is specifically designed for the quantitative detection of mouse FBXL3 levels in various sample types including serum, plasma, and tissue lysates. This ELISA kit offers high sensitivity and specificity, ensuring accurate and reliable results for your research needs. FBXL3 is a member of the F-box protein family and plays a key role in regulating the circadian clock and metabolism. Dysregulation of FBXL3 has been linked to various diseases including metabolic disorders, cancer, and neurodegenerative conditions, making it a valuable target for further investigation and potential therapeutic strategies.
With easy-to-follow instructions and a quick assay protocol, the Mouse FBXL3 ELISA Kit is a valuable tool for researchers studying the molecular mechanisms underlying circadian rhythm control, metabolic pathways, and disease pathogenesis. Invest in this kit today to advance your research in this important field.
Product Name:
Mouse F-Box And Leucine Rich Repeat Protein 3 (FBXL3) ELISA Kit
Product Code:
MODL00462
Size:
96 Assays
Target:
FBXL3
Synonyms:
FBL3, FBL3A, FBXL3A, F-box/LRR-repeat protein 3A
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
