The Mouse Fibrinogen-Like Protein 1 (FGL1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of FGL1 levels in mouse serum, plasma, and cell culture supernatants. This kit is ideal for researchers studying the role of FGL1 in various biological processes, including liver regeneration, immune response, and cancer development.FGL1 is a multifunctional protein that plays a critical role in regulating liver homeostasis and immune function.
It is also implicated in various diseases, such as liver cirrhosis and cancer, making it a valuable biomarker for monitoring disease progression and evaluating potential therapeutic interventions.With its reliable performance and ease of use, the Mouse FGL1 ELISA Kit provides researchers with a powerful tool for investigating the functions of FGL1 and exploring its potential as a therapeutic target. Order yours today and take your research to the next level!
Product Name:
Mouse Fibrinogen Like Protein 1 (FGL1) ELISA Kit
Product Code:
MODL00490
Size:
96 Assays
Target:
FGL1
Synonyms:
FGL-1, HFREP1, HP-041, LFIRE1, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, Liver fibrinogen-related protein 1
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
