Mouse Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit
The Mouse FNDC5 (Fibronectin Type III Domain-Containing Protein 5) ELISA Kit is a powerful tool for the precise measurement of FNDC5 levels in mouse samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for a variety of research applications.FNDC5 is a key regulator of metabolism and energy expenditure, as it is cleaved to produce irisin, a hormone that plays a role in improving glucose homeostasis and promoting brown fat activation.
Studies have shown that FNDC5/irisin has potential implications in metabolic diseases, obesity, and exercise physiology, highlighting its importance as a biomarker for investigations in these areas.Overall, the Mouse FNDC5 ELISA Kit is essential for researchers studying metabolic disorders, obesity, and exercise physiology, offering valuable insights into the role of FNDC5 in these conditions and its potential as a therapeutic target.
Product Name:
Mouse Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit
Product Code:
MODL00496
Size:
96 Assays
Target:
FNDC5
Synonyms:
FRCP2, Irisin, Fibronectin type III repeat-containing protein 2
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
