Mouse Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit
The Mouse Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit offered by Assay Genie is designed for the accurate detection of FBLIM1 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.FBLIM1 is a critical protein involved in cell adhesion, migration, and signal transduction, playing a key role in maintaining cell structure and function. Dysregulation of FBLIM1 has been linked to various diseases, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.
With its advanced technology and easy-to-follow protocol, the Mouse FBLIM1 ELISA Kit from Assay Genie is a must-have tool for researchers looking to delve deeper into the mechanisms of cell biology and disease progression. Get your hands on this kit today and unlock the key to unraveling the mysteries of FBLIM1 function.
Product Name:
Mouse Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit
Product Code:
MODL00458
Size:
96 Assays
Target:
FBLIM1
Synonyms:
CAL, FBLP-1, FBLP1, Migfilin, Mitogen-inducible 2-interacting protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
