The Mouse FIX (Coagulation Factor IX) ELISA Kit is specifically designed for the precise measurement of coagulation factor IX levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Coagulation factor IX, also known as Christmas factor, is a key player in the blood clotting cascade, facilitating the formation of fibrin clots and maintaining hemostasis. Dysregulation of factor IX can lead to bleeding disorders such as hemophilia B, highlighting the significance of its quantification in biomedical research. With the Mouse FIX ELISA Kit, researchers can investigate the role of coagulation factor IX in hemostasis, thrombosis, and other related processes in mouse models. This kit provides a valuable tool for studying coagulation disorders, evaluating treatment efficacy, and advancing our understanding of blood clotting mechanisms. Order now to enhance your research capabilities with reliable and accurate measurement of mouse FIX levels.
Product Name:
Mouse FIX (Coagulation Factor IX) ELISA Kit
SKU:
MOES00874
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
