The Mouse FLT3 Receptor (Type III Pro-Flt3) ELISA Kit is a reliable and accurate tool for the quantitative detection of FLT3 receptor levels in mouse samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it perfect for various research applications. The FLT3 receptor, also known as Fms-like tyrosine kinase 3, is a crucial protein involved in the regulation of hematopoietic stem cell proliferation and differentiation. Abnormalities in FLT3 receptor signaling have been associated with leukemia and other hematological malignancies, making it a valuable biomarker for studying these diseases and developing potential targeted therapies.
This ELISA kit provides researchers with a powerful tool to accurately measure FLT3 receptor levels in mouse samples, enabling a better understanding of its role in normal and diseased conditions. By using this kit, researchers can uncover valuable insights into the biology and pathology of FLT3 receptor-related disorders.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-103%
92-104%
89-103%
EDTA Plasma (n = 5)
85-96%
87-96%
85-100%
Heparin Plasma (n = 5)
85-100%
81-99%
82-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-103
94
EDTA Plasma (n = 5)
85-104
97
Heparin Plasma (n = 5)
89-105
98
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
