The Mouse FXII (Coagulation Factor XII) CLIA Kit is specifically designed for the quantitative measurement of FXII levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it ideal for various research applications.FXII, also known as Hageman factor, is a key player in the coagulation cascade and is involved in blood clot formation. Dysregulation of FXII activity has been linked to thrombotic disorders and inflammatory conditions, highlighting its importance as a potential biomarker for studying these diseases and developing targeted treatments.
This kit offers a straightforward and efficient method for measuring FXII levels, allowing researchers to investigate the role of FXII in various physiological and pathological processes with confidence. Trust the Mouse FXII CLIA Kit from Assay Genie for precise and reproducible results in your research endeavors.
Product Name:
Mouse FXII (Coagulation Factor XII) CLIA Kit
SKU:
MOES00186
Target:
Mouse FXII (Coagulation Factor XII)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse F12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse F12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse F12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse F12. You can calculate the concentration of Mouse F12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
100-118
88-100
Average (%)
107
108
93
1:4
Range (%)
99-115
99-116
89-99
Average (%)
105
107
94
1:8
Range (%)
90-104
91-105
87-98
Average (%)
98
97
93
1:16
Range (%)
89-103
104-121
99-115
Average (%)
96
110
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
84-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.16
183.72
730.37
104.63
179.23
800.53
Standard deviation
9.58
19.93
45.87
12.31
16.24
69.89
C V (%)
9.56
10.85
6.28
11.77
9.06
8.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse F12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse F12 in samples. No significant cross-reactivity or interference between Mouse F12 and analogues was observed.
