The Mouse GH (Growth Hormone) CLIA Kit is specifically designed for the precise quantification of growth hormone levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for a variety of research purposes.Growth hormone is a key regulator of growth, metabolism, and development in mammals, playing a crucial role in various physiological processes. Dysregulation of growth hormone levels can lead to disorders such as dwarfism, gigantism, and metabolic abnormalities, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions. The Mouse GH CLIA Kit from Assay Genie provides researchers with a reliable tool for measuring growth hormone levels in mouse samples, facilitating in-depth investigations into the mechanisms underlying growth and development. Trust in our kit to deliver accurate and reliable results for your research needs.
Product Name:
Mouse GH (Growth Hormone) CLIA Kit
SKU:
MOES00052
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
