The Mouse Ghrelin ELISA Kit is specifically designed for the precise measurement of ghrelin levels in mouse serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it an ideal tool for various research applications. Ghrelin is a key hormone that regulates appetite and energy balance, playing a crucial role in metabolic processes and weight regulation. It is also involved in the regulation of growth hormone release and gastric motility, making it a valuable biomarker for studying obesity, eating disorders, and metabolic diseases. By utilizing the Mouse Ghrelin ELISA Kit, researchers can effectively study the role of ghrelin in various physiological and pathological conditions, paving the way for potential therapeutic strategies targeting ghrelin signaling pathways.
Product Name:
Mouse GHRL (Ghrelin) ELISA Kit
SKU:
MOES01068
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
