The Mouse HDL (High Density Lipoprotein) ELISA Kit is specially designed for the precise measurement of HDL levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results, which makes it perfect for various research purposes.HDL is an important component involved in lipid metabolism, playing a vital role in cardiovascular health by removing excess cholesterol from the bloodstream. Monitoring HDL levels is crucial in understanding the risk of heart disease and other related conditions. This ELISA kit provides a reliable method for studying HDL levels in mouse models, offering valuable insights into lipid metabolism and potential therapeutic strategies.
Product Name:
Mouse HDL (High Density Lipoprotein) ELISA Kit
SKU:
MOES01676
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 nmol/mL
Detection range:
3.13-200 nmol/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
