The Mouse Hepcidin ELISA Kit from Assay Genie is a powerful tool for detecting levels of hepcidin in mouse samples. Hepcidin is a key regulator of iron metabolism, playing a crucial role in conditions such as anemia, hemochromatosis, and iron deficiency disorders.This kit offers high sensitivity and specificity, ensuring accurate and reliable results in serum, plasma, and cell culture supernatants. By measuring hepcidin levels, researchers can gain valuable insights into iron regulation and its impact on various health conditions.The Mouse Hepcidin ELISA Kit from Assay Genie is essential for studying iron metabolism and its implications for health and disease. Get reliable and reproducible results for your research with this advanced kit.
Product Name:
Mouse Hepc (Hepcidin) ELISA Kit
SKU:
MOES01173
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
75 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
