Mouse High Affinity Choline Transporter (ChT) ELISA Kit
The Mouse High Affinity Choline Transporter (CHT) ELISA Kit is a powerful tool for measuring levels of CHT in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for accurate and consistent results that are perfect for a variety of research applications.CHT is a key protein involved in choline transport, an essential process for neurotransmitter synthesis and brain function.
Dysregulation of CHT has been linked to neurological disorders such as Alzheimer's disease, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With the Mouse High Affinity Choline Transporter (CHT) ELISA Kit, researchers can confidently measure CHT levels in mouse samples, gaining valuable insight into the role of this protein in neuronal health and disease.
Product Name:
Mouse High Affinity Choline Transporter (ChT) ELISA Kit
Product Code:
MODL00269
Size:
96 Assays
Target:
ChT
Synonyms:
SLC5A7, hCHT, CHT1, Solute Carrier Family 5 Member 7,Choline Transporter, Hemicholinium-3-sensitive choline transporter
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
85-101
93
EDTA plasma (n=5)
86-98
92
Heparin plasma (n=5)
87-101
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
