SynonymsEPAS1,HLF,MOP2,PASD2,BHLHE73,Endothelial PAS Domain 1,HIF-1 Alpha-Like Factor,Basic-helix-loop-helix-PAS protein MOP2,Class E basic helix-loop-helix protein 73
ReactivityMouse
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
MODL00621
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97481
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
EPAS1,HLF,MOP2,PASD2,BHLHE73,Endothelial PAS Domain 1,HIF-1 Alpha-Like Factor,Basic-helix-loop-helix-PAS protein MOP2,Class E basic helix-loop-helix protein 73
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Mouse Hypoxia Inducible Factor 2 Alpha (HIF2A) ELISA Kit is a powerful tool for the accurate measurement of HIF2A levels in mouse samples including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing researchers with reliable and reproducible results for a variety of research applications.HIF2A is a key transcription factor that plays a crucial role in the cellular response to hypoxia, regulating a wide range of genes involved in oxygen homeostasis.
Dysregulation of HIF2A has been implicated in various diseases including cancer, cardiovascular disorders, and metabolic diseases, highlighting its significance as a potential therapeutic target.With the Mouse HIF2A ELISA Kit, researchers can easily quantify HIF2A levels in mouse samples, paving the way for a better understanding of its role in disease pathogenesis and the development of novel treatment strategies.
EPAS1, HLF, MOP2, PASD2, BHLHE73, Endothelial PAS Domain 1, HIF-1 Alpha-Like Factor, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
