Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
The Mouse IGFBP5 (Insulin-like Growth Factor Binding Protein 5) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of IGFBP5 levels in mouse serum, plasma, and cell culture supernatants. This kit ensures reliable and reproducible results, making it suitable for a variety of research applications.IGFBP5 is a key regulator of insulin-like growth factor signaling, influencing cell growth, survival, and differentiation. It plays a crucial role in various physiological processes and has been implicated in diseases such as cancer, diabetes, and osteoporosis. The Mouse IGFBP5 ELISA Kit is a valuable tool for studying the role of IGFBP5 in these conditions and exploring potential therapeutic interventions.
Product Name:
Mouse IGFBP5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit
SKU:
MOES01739
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
75 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
