The Mouse Immunoglobulin G1 (IgG1) ELISA Kit is meticulously crafted for the precise quantitative measurement of IgG1 levels in a variety of biological samples. Immunoglobulin G1 is a prominent antibody subclass known for its crucial role in mediating immune responses, including pathogen recognition, opsonization, and antigen neutralization. This ELISA kit provides exceptional sensitivity and specificity, facilitating accurate determination of IgG1 levels and furthering the understanding of its immunological functions. Manufactured under rigorous quality control standards, the kit ensures robust and reproducible results, making it an ideal tool for research endeavors aiming to explore the intricate mechanisms of immune responses governed by IgG1 antibodies.
Product Name:
Mouse IgG1 (Immunoglobulin G1) ELISA Kit
SKU:
AEES00230
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
