The Mouse IL-13 CLIA Kit is specifically designed for the quantitative detection of Interleukin-13 (IL-13) levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.IL-13 is a key cytokine involved in regulating immune responses, particularly in allergic and inflammatory conditions. It plays a crucial role in modulating inflammation, airway hyperresponsiveness, and tissue remodeling, making it a valuable biomarker for studying conditions such as asthma, atopic dermatitis, and autoimmune diseases.
By measuring IL-13 levels with precision, researchers can gain insights into disease mechanisms, monitor treatment responses, and identify potential therapeutic targets. The Mouse IL-13 CLIA Kit provides a powerful tool for advancing research in immunology, inflammation, and respiratory diseases.
Product Name:
Mouse IL-13 CLIA Kit
SKU:
MOES00408
Target:
Mouse IL-13
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-13. You can calculate the concentration of Mouse IL-13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
94-109
86-100
Average (%)
106
101
93
1:4
Range (%)
92-104
97-114
84-98
Average (%)
97
104
91
1:8
Range (%)
103-115
86-99
87-100
Average (%)
109
91
93
1:16
Range (%)
86-101
86-98
93-108
Average (%)
93
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
92-107
100
Cell culture media (n=5)
95-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.38
128.32
267.48
43.07
128.03
275.46
Standard deviation
4.53
12.02
28.97
4.91
14.34
28.37
C V (%)
10.44
9.37
10.83
11.4
11.2
10.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-13 in samples. No significant cross-reactivity or interference between Mouse IL-13 and analogues was observed.
