The Mouse IL-18 ELISA Kit is a powerful tool for measuring levels of interleukin-18 in mouse serum, plasma, and tissue culture samples. This kit offers superior sensitivity and specificity, providing accurate and consistent results for a variety of research applications.Interleukin-18, also known as IL-18, is a key cytokine involved in the immune response, inflammation, and regulation of cell growth. It plays a critical role in various physiological processes and is implicated in several disease states, including autoimmune disorders, infectious diseases, and inflammatory conditions.
By using the Mouse IL-18 ELISA Kit, researchers can gain valuable insights into the role of interleukin-18 in disease pathology and potentially identify new therapeutic targets. With its reliable performance and ease of use, this kit is an essential tool for advancing our understanding of immune regulation and inflammation in mouse models.
Product Name:
Mouse IL-18 ELISA Kit
SKU:
MOES01225
Target:
Mouse IL-18
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-18. You can calculate the concentration of Mouse IL-18 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
87-101
86-99
Average (%)
97
92
93
1:4
Range (%)
97-109
81-93
94-110
Average (%)
104
87
101
1:8
Range (%)
96-109
80-95
97-110
Average (%)
101
87
103
1:16
Range (%)
96-109
88-99
96-112
Average (%)
101
93
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.5
145.2
342.8
53.0
144.6
363.4
Standard deviation
2.6
8.1
16.8
3.7
7.4
12.4
C V (%)
5.25
5.58
4.9
6.98
5.12
3.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IL-18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-18 in samples. No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed.
