Mouse IL1B Recombinant Protein (RPPB0460)
- SKU:
- RPPB0460
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- P10749
- Research Area:
- Cytokines
Description
| Product Name: | Mouse IL1B Recombinant Protein |
| Product Code: | RPPB0460 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | IL1B |
| Synonyms: | Interleukin 1 beta, IL-1b, IL-1beta, Catabolin, H1, IL 1,IL 1 beta,IL-1 beta, IL1 BETA,IL1B,IL1B_HUMAN,IL1F2, Interleukin 1 beta, Interleukin-1 beta, OAF,OTTHUMP00000162031, Preinterleukin 1 beta,Pro interleukin 1 beta. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | IL1B protein (1mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF LGNNSGQDII DFTMESVSS |
| Biological Activity: | Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range < 0.1 ng/ml. |
Interleukin-1 beta is a cytokine that causes inflammation and can regulate angiogenesis through interaction with vascular endothelial cells or promoting the creation of proangiogenic modulators through the paracrine system. The cytokine causes migration and proliferation of endothelial cells, creation of mediators to inflammation, expression of adhesion-molecules & recruit of leukocyte cells. Interleukin-1 beta was found crucial for the process of tumors in various organism models.��
IL1B Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids (118-269 a.a) and having a molecular mass of 21kDa.IL1B is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. |
| UniProt Protein Details: | Protein type:Cytokine Cellular Component: extracellular space; cell; extracellular region; intracellular; cytosol; vesicle; secretory granule Molecular Function:protein domain specific binding; interleukin-1 receptor binding; cytokine activity; receptor binding Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of JNK activity; negative regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; negative regulation of glutamate secretion; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; positive regulation of glial cell differentiation; positive regulation of lipid catabolic process; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; fever; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; positive regulation of interleukin-6 production; interleukin-1 beta production; social behavior; positive regulation of angiogenesis; negative regulation of neuron differentiation; positive regulation of cell division; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of lipid metabolic process; leukocyte migration; sequestering of triacylglycerol; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; response to ATP; memory; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of fever; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion |
| NCBI Summary: | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P10749 |
| NCBI GenInfo Identifier: | 124304 |
| NCBI Gene ID: | 16176 |
| NCBI Accession: | P10749.1 |
| UniProt Secondary Accession: | P10749,Q2M4J6, |
| UniProt Related Accession: | P10749 |
| Molecular Weight: | 30,931 Da |
| NCBI Full Name: | Interleukin-1 beta |
| NCBI Synonym Full Names: | interleukin 1 beta |
| NCBI Official Symbol: | Il1b�� |
| NCBI Official Synonym Symbols: | Il-1b; IL-1beta�� |
| NCBI Protein Information: | interleukin-1 beta; IL-1 beta |
| UniProt Protein Name: | Interleukin-1 beta |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il1b�� |
| UniProt Entry Name: | IL1B_MOUSE |
