The Mouse IL-2 CLIA Kit is a reliable and accurate tool for detecting levels of interleukin-2 (IL-2) in mouse serum, plasma, and cell culture supernatants. This high sensitivity and specificity kit ensures precise and reproducible results, making it perfect for a variety of research applications.IL-2 is a key cytokine involved in regulating the immune response, including the proliferation and differentiation of T cells.
Dysregulation of IL-2 levels has been linked to autoimmune disorders, inflammatory diseases, and cancer, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.Overall, the Mouse IL-2 CLIA Kit is a valuable asset for researchers looking to study the immune system and its associated diseases in mouse models.
Product Name:
Mouse IL-2 CLIA Kit
SKU:
MOES00039
Target:
Mouse IL-2
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-2. You can calculate the concentration of Mouse IL-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
93-106
91-104
Average (%)
105
99
97
1:4
Range (%)
85-97
90-105
84-97
Average (%)
92
97
91
1:8
Range (%)
91-107
98-116
100-114
Average (%)
98
106
108
1:16
Range (%)
90-102
90-106
91-104
Average (%)
95
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.7
76.66
200.94
28.08
73.61
214.66
Standard deviation
2.52
5.41
21.42
2.72
6.12
18.61
C V (%)
9.44
7.06
10.66
9.69
8.31
8.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-2 in samples. No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed.
