The Mouse IL-5 ELISA Kit is a valuable tool for researchers looking to accurately measure interleukin-5 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research pursuits.Interleukin-5 is a key cytokine involved in the regulation of immune responses, particularly in the differentiation and activation of eosinophils. It plays a critical role in conditions such as asthma, allergies, and autoimmune diseases, making it a crucial biomarker for studying these conditions and exploring potential treatment options.With its user-friendly design and reliable performance, the Mouse IL-5 ELISA Kit is an essential tool for any researcher looking to delve deeper into the role of interleukin-5 in immune regulation and disease pathology.
Product Name:
Mouse IL-5 ELISA Kit
SKU:
MOES01217
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
