The Mouse IL-6 ELISA Kit is a highly sensitive and specific assay designed to accurately measure interleukin-6 levels in mouse serum, plasma, and tissue culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.Interleukin-6 is a key cytokine involved in inflammation and immune response regulation. It plays a critical role in various physiological processes, including the immune system, hematopoiesis, and acute phase responses. Abnormal levels of IL-6 have been associated with a wide range of diseases, including inflammatory disorders, autoimmune diseases, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. Overall, the Mouse IL-6 ELISA Kit is a valuable tool for researchers looking to investigate the role of interleukin-6 in different biological pathways and diseases in mouse models. Its high sensitivity and specificity ensure accurate and reliable results, enabling precise measurements of IL-6 levels for a better understanding of its biological functions.
Product Name:
Mouse IL-6 ELISA Kit
SKU:
MOES00663
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
