Mouse Insulin Like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit
The Mouse Insulin-like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit is specifically designed for the accurate quantification of IGFBP2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.IGFBP2 is a key regulator of insulin-like growth factor signaling and is involved in cell growth, differentiation, and survival. Aberrant expression of IGFBP2 has been associated with various diseases, including cancer, metabolic disorders, and neurological conditions, making it a valuable biomarker for understanding these diseases and developing potential treatments.
This ELISA kit provides researchers with a powerful tool for studying the role of IGFBP2 in health and disease, offering insights into its biological functions and its potential as a therapeutic target. With its easy-to-use format and accurate results, the Mouse IGFBP2 ELISA Kit is an essential tool for any laboratory conducting research in this field.
Product Name:
Mouse Insulin Like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit
Product Code:
MODL00664
Size:
96 Assays
Target:
IGFBP2
Synonyms:
IBP2, IGF-BP53
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
