Mouse Inter Alpha-Globulin Inhibitor H5 (ITIH5) ELISA Kit
The Mouse Inter-Alpha Globulin Inhibitor H5 (ITIH5) ELISA Kit is specifically designed for the accurate measurement of ITIH5 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results, making it ideal for a wide range of research applications.ITIH5 is a key protein involved in the inhibition of angiogenesis, playing a crucial role in regulating blood vessel formation and cell proliferation. Dysregulation of ITIH5 has been linked to various diseases such as cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for understanding these diseases and developing potential therapeutic strategies.
With the Mouse Inter-Alpha Globulin Inhibitor H5 ELISA Kit, researchers can accurately quantify ITIH5 levels in mouse samples, providing valuable insights into the role of this protein in various physiological and pathological processes. Get reliable and consistent results with this advanced ELISA kit from AssayGenie.
Product Name:
Mouse Inter Alpha-Globulin Inhibitor H5 (ITIH5) ELISA Kit
Product Code:
MODL00749
Size:
96 Assays
Target:
ITIH5
Synonyms:
Inter-alpha-trypsin inhibitor heavy chain H5
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
