SynonymsIHRP,PK120,H4P,ITIHL1,Plasma Kallikrein-Sensitive Glycoprotein,Plasma kallikrein sensitive glycoprotein 120,Inter-alpha-trypsin inhibitor family heavy chain-related protein
ReactivityMouse
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
MODL00748
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
A6X935
Sensitivity:
33ng/mL
Range:
78.12-5000ng/mL
ELISA Type:
Sandwich
Synonyms:
IHRP,PK120,H4P,ITIHL1,Plasma Kallikrein-Sensitive Glycoprotein,Plasma kallikrein sensitive glycoprotein 120,Inter-alpha-trypsin inhibitor family heavy chain-related protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Mouse Inter-alpha-trypsin Inhibitor Heavy Chain H4 (ITIH4) ELISA Kit is a reliable tool for precise measurement of ITIH4 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ITIH4 is a protein that plays a role in inflammation and immune response regulation. Elevated levels of ITIH4 have been associated with various diseases, including liver cirrhosis, cancer, and inflammatory conditions.
This ELISA kit provides researchers with a valuable tool for studying these conditions and exploring potential therapeutic targets.With its user-friendly protocol and high performance characteristics, the Mouse ITIH4 ELISA Kit is an essential tool for researchers seeking to investigate the role of ITIH4 in health and disease.
Product Name:
Mouse ITIH4 ELISA Kit
Product Code:
MODL00748
Size:
96 Assays
Target:
ITIH4
Synonyms:
IHRP, PK120, H4P, ITIHL1, Plasma Kallikrein-Sensitive Glycoprotein, Plasma kallikrein sensitive glycoprotein 120, Inter-alpha-trypsin inhibitor family heavy chain-related protein
Detection Method:
Sandwich
Range:
78.12-5,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
