The Mouse Interleukin-20 (IL-20) ELISA Kit is a reliable and sensitive assay designed for the accurate detection of IL-20 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and sensitivity, ensuring consistent and reproducible results for a variety of research applications.IL-20 is a cytokine that plays a crucial role in inflammatory processes and immune responses. It is involved in various physiological and pathological conditions, including autoimmune diseases, skin disorders, and cancer.
Monitoring IL-20 levels can provide valuable insights into disease progression and the development of potential therapeutic strategies.With its high performance and ease of use, the Mouse Interleukin-20 (IL-20) ELISA Kit is an indispensable tool for researchers studying the complex mechanisms of cytokine signaling and its implications in various disease states. Trust in this kit to deliver accurate and dependable results for your research needs.
Product Name:
Mouse Interleukin 20 (IL-20) ELISA Kit
Product Code:
MODL00697
Size:
96 Assays
Target:
IL20
Synonyms:
IL10D, IL10-D, ZCYTO10, Cytokine Zcyto10
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
