The Mouse Interleukin-8 Receptor Alpha (IL8RA) ELISA Kit is specifically designed for the precise measurement of IL8RA levels in mouse samples such as serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it a reliable tool for a variety of research applications.IL8RA is a vital receptor involved in the immune response, inflammation, and chemotaxis processes. It plays a crucial role in various diseases and conditions, including autoimmune disorders, inflammatory diseases, and cancer.
Studying IL8RA levels can provide valuable insights into the underlying mechanisms of these conditions and aid in the development of potential therapeutic strategies.Overall, the Mouse IL8RA ELISA Kit is an essential tool for researchers looking to investigate the role of IL8RA in immune responses, inflammation, and disease pathology in mouse models. Its high performance and ease of use make it a valuable asset in the study of this important receptor.
CD181, CDw128a, CXCR1, CKR1, CMKAR1, IL8R1, IL8RBA, IL8-RA, Chemokine C-X-C motif Receptor 1, High Affinity Interleukin-8 Receptor A
Detection Method:
Sandwich
Range:
0.156-10 ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
